Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay
نویسندگان
چکیده
منابع مشابه
Taqman real-time PCR assay based on ORFV024 gene for rapid detection of orf infection.
In this study, a TaqMan real-time polymerase chain reaction (PCR) assay was developed to detect and quantify orf virus (ORFV) DNA in infected cell culture and clinical samples. Primers and probes were designed to amplify an 87 bp fragment DNA based on the sequence of ORFV024 gene encoding an NF-κB inhibitor of orf virus. The assay was highly specific and sensitive for ORFV DNA and no cross-reac...
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The objective of this study was to develop and validate a Taqman real-time PCR assay for the detection of Mycoplasma bovis (M. bovis). Unique primers targeting the highly conserved house-keeping gene (uvrC) were designed and the probe sequence was derived from a previously published microarray study. There was 100% agreement in the outcome between our assay and the other two published assays fo...
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ژورنال
عنوان ژورنال: BMC Veterinary Research
سال: 2018
ISSN: 1746-6148
DOI: 10.1186/s12917-018-1600-3